Journal: Scientific Reports

Article Title: Tissue-specific Differentiation Potency of Mesenchymal Stromal Cells from Perinatal Tissues

doi: 10.1038/srep23544

Figure Lengend Snippet: ( A ) Morphology of amnionic mesenchymal stromal cells(AMSCs) at primary culture, passage 3 and 6 (left), chorionic MSCs (CMSCs) (middle), and umbilical cord MSCs (UC-MSCs) (right) in primary culture, passage 3 and 10 (x40). ( B ) Comparison of population doubling time among AMSCs, CMSCs and UC-MSCs. ( C ) Evaluation of apoptosis by Annexin V-FITC/PI staining, followed by flow cytometry analysis. ( D ) Evaluation of Senescence by β-galactosidase activity assay. ( E ) Mesodermal differentiation potential of mesenchymal stromal cells derived from amnion (AMSCs), chorion (CMSCs) and umbilical cord (UC-MSCs). Adipogenesis (Oil red O), chondrogenesis (Alcian blue), osteogenesis (silver nitrate) (x100).

Article Snippet: Sections were stained with 1% alcian blue solution (ScienCell, Carlsbad, CA).

Techniques: Comparison, Staining, Flow Cytometry, Activity Assay, Derivative Assay

Journal: Communications Biology

Article Title: Mandarin fish (Sinipercidae) genomes provide insights into innate predatory feeding

doi: 10.1038/s42003-020-1094-y

Figure Lengend Snippet: a Number of zebrafish gill rakers (arrowheads, 0.1% alcian blue staining) at 2, 3 and 8 dph. b Zebrafish edar expression assessed by whole-mount in situ hybridization. Control fish or fish treated with 1 ng/ml activin A or 50 ng/ml BMP4 at 2, 3, 8 and dph and the negative control (N-Ctrl) are shown. c Zebrafish larvae intake of brine shrimp. d The distance between gill rakers of zebrafish at 8 dph ( n = 3). e Zebrafish feeding at 8 dph. The numbers of fish that ingested 5-day-old shrimp were shown ( n = 20). The boxplot elements are defined as: center line, median; box limits, the third and first quartiles; whiskers, 1.5× interquartile range. * indicates a significant difference ( P < 0.05).

Article Snippet: For gill raker staining, the larvae were stained with 0.1% alcian blue (Sinopharm Chemical Reagent Co.) as previously described.

Techniques: Staining, Expressing, In Situ Hybridization, Control, Negative Control

Journal: Stem Cell Research & Therapy

Article Title: Isolation and characterisation of nasoseptal cartilage stem/progenitor cells and their role in the chondrogenic niche

doi: 10.1186/s13287-020-01663-1

Figure Lengend Snippet: Chondrogenic potential of CDC, DNC and CSPC populations. a Relative gene expression of chondrogenic-, proliferative- and progenitor-related genes in CDCs, DNCs and CSPCs. Expression levels were normalised to the level of RPLP0 , and relative expression was calculated against CDCs. CCND2 , cell cycle cyclin D2; BMP2 , bone morphogenetic protein 2; COL1A1 , type 1 collagen; COL2A1 , type 2 collagen; ACAN , aggrecan; CXCL12, C-X-C motif chemokine 12; NCAM1 , neural cell adhesion molecule 1; SOX9 , sex-determining region Y box 9. Results as mean ± SEM from 6 independent experiments ( n = 6) in technical triplicates, * p < 0.05, ** p < 0.01 and *** p < 0.001. b – j Histology staining of b – d CDCs, e – g DNCs and h – j CSPCs at P2 using b , e and h Alcian blue; c , f and i Safranin-O; and d , g and j toluidine blue. Images are representative from 3 independent experiments ( n = 3). Scale bars, 100 μm

Article Snippet: Cells were stained with 1% Alcian blue (TCS Biosciences, Buckingham, UK) solution for 15 min and washed with water, while others were stained with 0.1% toluidine blue (TCS Biosciences) solution in water for 3 min following wash with water.

Techniques: Gene Expression, Expressing, Staining